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human pdgfaa  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec human pdgfaa
    Human Pdgfaa, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pdgfaa/Human+PDGF-AA%2C+research+grade/pm40728770-70-31-37
    Average 93 stars, based on 8 article reviews
    human pdgfaa - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods.
    Article Snippet: After the last wash, columns were removed from a magnetic field, 1 ml of culture medium was applied and using the plunger, cells labelled with A2B5 magnetic microbeads (positive fraction) were removed into an eppendorf tube. .. Culture medium (“PROLIF”) consisted of: MACS Neuro Medium (Miltenyi), 0.5 mM l-glutamine (Sigma), 1% ITS (Gibco), 1% AAS (Sigma-Aldrich), 10 ng/ ml Human PDGFAA (Miltenyi), 10 ng/ml Mouse FGF-1 (Miltenyi). ..

    Article Title: Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods
    Article Snippet: After the last wash, columns were removed from a magnetic field, 1 ml of culture medium was applied and using the plunger, cells labelled with A2B5 magnetic microbeads (positive fraction) were removed into an eppendorf tube. .. Culture medium (“PROLIF”) consisted of: MACS Neuro Medium (Miltenyi), 0.5 mM l -glutamine (Sigma), 1% ITS (Gibco), 1% AAS (Sigma-Aldrich), 10 ng/ml Human PDGFAA (Miltenyi), 10 ng/ml Mouse FGF-1 (Miltenyi). ..

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia
    Article Snippet: Columns were washed with the PBSA solution (0.5% BSA [Bovine serum albumin; Sigma] in PBS [Gibco]) and taken from the magnetic field to allow the retrieval of A2B5-labeled cells (positive fraction). .. Obtained cells were cultured in medium allowing OPC proliferation, which consisted of MACS Neuro Medium (Miltenyi), 0.5 mM l -glutamine (Sigma-Aldrich), 1% ITS (insulin-transferrin-selenium; Gibco), 1% AAS (antibiotic–antimycotic solution; Sigma-Aldrich), 10 ng/ml human PDGFAA (platelet-derived growth factor AA; Miltenyi), and 10 ng/ml mouse FGF-1 (fibroblast growth factor 1; Miltenyi). ..

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia.
    Article Snippet: Preterm or complicated labor is often accompanied by the asphyxia of the newborn.. Temporal limitation of oxygen and nutrient supply can be especially harmful to the developing brain and lead to hypoxic-ischemic encephalopathy.. This injury is mainly caused by the reduction in white matter volume and may be triggered by delayed maturation of oligodendrocytes and affected myelination.

    Atomic Absorption Spectroscopy:

    Article Title: Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods.
    Article Snippet: After the last wash, columns were removed from a magnetic field, 1 ml of culture medium was applied and using the plunger, cells labelled with A2B5 magnetic microbeads (positive fraction) were removed into an eppendorf tube. .. Culture medium (“PROLIF”) consisted of: MACS Neuro Medium (Miltenyi), 0.5 mM l-glutamine (Sigma), 1% ITS (Gibco), 1% AAS (Sigma-Aldrich), 10 ng/ ml Human PDGFAA (Miltenyi), 10 ng/ml Mouse FGF-1 (Miltenyi). ..

    Article Title: Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods
    Article Snippet: After the last wash, columns were removed from a magnetic field, 1 ml of culture medium was applied and using the plunger, cells labelled with A2B5 magnetic microbeads (positive fraction) were removed into an eppendorf tube. .. Culture medium (“PROLIF”) consisted of: MACS Neuro Medium (Miltenyi), 0.5 mM l -glutamine (Sigma), 1% ITS (Gibco), 1% AAS (Sigma-Aldrich), 10 ng/ml Human PDGFAA (Miltenyi), 10 ng/ml Mouse FGF-1 (Miltenyi). ..

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia
    Article Snippet: Columns were washed with the PBSA solution (0.5% BSA [Bovine serum albumin; Sigma] in PBS [Gibco]) and taken from the magnetic field to allow the retrieval of A2B5-labeled cells (positive fraction). .. Obtained cells were cultured in medium allowing OPC proliferation, which consisted of MACS Neuro Medium (Miltenyi), 0.5 mM l -glutamine (Sigma-Aldrich), 1% ITS (insulin-transferrin-selenium; Gibco), 1% AAS (antibiotic–antimycotic solution; Sigma-Aldrich), 10 ng/ml human PDGFAA (platelet-derived growth factor AA; Miltenyi), and 10 ng/ml mouse FGF-1 (fibroblast growth factor 1; Miltenyi). ..

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia.
    Article Snippet: Preterm or complicated labor is often accompanied by the asphyxia of the newborn.. Temporal limitation of oxygen and nutrient supply can be especially harmful to the developing brain and lead to hypoxic-ischemic encephalopathy.. This injury is mainly caused by the reduction in white matter volume and may be triggered by delayed maturation of oligodendrocytes and affected myelination.

    Cell Culture:

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia
    Article Snippet: Columns were washed with the PBSA solution (0.5% BSA [Bovine serum albumin; Sigma] in PBS [Gibco]) and taken from the magnetic field to allow the retrieval of A2B5-labeled cells (positive fraction). .. Obtained cells were cultured in medium allowing OPC proliferation, which consisted of MACS Neuro Medium (Miltenyi), 0.5 mM l -glutamine (Sigma-Aldrich), 1% ITS (insulin-transferrin-selenium; Gibco), 1% AAS (antibiotic–antimycotic solution; Sigma-Aldrich), 10 ng/ml human PDGFAA (platelet-derived growth factor AA; Miltenyi), and 10 ng/ml mouse FGF-1 (fibroblast growth factor 1; Miltenyi). ..

    Article Title: Inhibition of the HIF-1α Signaling Pathway Promotes Oligodendrocyte Maturation in the In Vitro Model of Perinatal Asphyxia.
    Article Snippet: Preterm or complicated labor is often accompanied by the asphyxia of the newborn.. Temporal limitation of oxygen and nutrient supply can be especially harmful to the developing brain and lead to hypoxic-ischemic encephalopathy.. This injury is mainly caused by the reduction in white matter volume and may be triggered by delayed maturation of oligodendrocytes and affected myelination.



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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    Miltenyi Biotec human pdgfaa
    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
    Human Pdgfaa, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Image Search Results


    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Journal: bioRxiv

    Article Title: INPP5E interactome reveals novel connections to growth factor signaling

    doi: 10.64898/2026.02.13.705725

    Figure Lengend Snippet: (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Article Snippet: Other reagents included GFP-Trap_MA beads (Proteintech, gtma), mouse anti-Flag M2 magnetic beads (Sigma, M8823), sodium orthovanadate (Alfa Aesar, J60191) and the following recombinant human growth factors: PDGF-AA (Proteintech, HZ-1215), TGF-β1 (R&D Systems, 240-B), Insulin (Gibco, 12585014), IGF-I (Proteintech, HZ-1322) and EGF (Proteintech, HZ-1326).

    Techniques: Western Blot, Control, Cell Culture, Marker